Journal: Experimental & Molecular Medicine

Article Title: TFAP2C-mediated upregulation of TGFBR1 promotes lung tumorigenesis and epithelial–mesenchymal transition

doi: 10.1038/emm.2016.125

Figure Lengend Snippet: TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 VA4 cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.

Article Snippet: The human NSCLC cell lines, NCI-H292 and NCI-H838, and the human normal lung cell lines, MRC5 and WI-26 VA4, were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA), authenticated and maintained in early passages, no more than 6 months after receipt from ATCC.

Techniques: Binding Assay, Knockdown, Small Interfering RNA, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal:

Article Title: HtrA Homologue of Legionella pneumophila : an Indispensable Element for Intracellular Infection of Mammalian but Not Protozoan Cells

doi: 10.1128/IAI.69.4.2569-2579.2001

Figure Lengend Snippet: Intracellular growth kinetics of GT20 and the parental strain AA100 in (a) U937 macrophage-like cells, (b) WI-26 alveolar epithelial cells, and (c) A. polyphaga. Strain GT20(pLP102) is a plasmid-complemented clone of GT20. These data are representative of at least three independent experiments performed in triplicate. The absence of error bars indicates very small standard deviations that could not be displayed.

Article Snippet: Macrophage-like U937 cells and human type I alveolar epithelial cells (WI-26 VA4; ATCC CCL-95.1) and axenic A. polyphaga were maintained and used for infections as described previously ( 26 , 27 ).

Techniques: Plasmid Preparation

Journal:

Article Title: HtrA Homologue of Legionella pneumophila : an Indispensable Element for Intracellular Infection of Mammalian but Not Protozoan Cells

doi: 10.1128/IAI.69.4.2569-2579.2001

Figure Lengend Snippet: Cytopathogenic defect of GT20 is due to the defect in intracellular replication. (a) Cytopathogenicity of GT20 and the parental strain AA100 to U937 macrophage-like cells as determined by Alamar Blue dye reduction at various time points following infection at an MOI of 0.5. Strain GT20(pLP102) is a plasmid-complemented clone of GT20. Percent killing was normalized to uninfected cells, which were considered 100% viable. These data are representative of at least three independent experiments performed in triplicate. (b) Induction of apoptosis as evidenced by DNA fragmentation in U937 macrophages following 3 h of incubation after 1 h of infection at an MOI of 50. The φχ size standard is shown on the left. (c) Contact-dependent hemolysis of sheep red blood cells following 1 h of incubation at a bacterium-blood cell ratio of 25:1. Hemolysis was measured spectrophotometrically at A415. RBC represents red blood cells incubated in the absence of bacteria. These data are representative of at least three independent experiments performed in triplicate. The absence of error bars indicates very small standard deviations that could not be displayed.

Article Snippet: Macrophage-like U937 cells and human type I alveolar epithelial cells (WI-26 VA4; ATCC CCL-95.1) and axenic A. polyphaga were maintained and used for infections as described previously ( 26 , 27 ).

Techniques: Infection, Plasmid Preparation, Incubation